Signalling

Part:BBa_K233307:Design

Designed by: Swetha Srinivasan, Samit Watve, Mandar Phatak, Chinar Patil   Group: iGEM09_IBB_Pune   (2009-10-16)

TorA- This part is a export tag that utilizes the Twin Arginine Transport pathway(TAT)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was originally designed by Basudeb Bhattacharyya of the Wisocnsin 2008 iGEM team. We contacted them for more information and obtained a suitable reference for the same.

This set of tags utilizes the Twin Arginine Translocation pathway(TAT)and need to be fused at the 5' end of the gene whose protein is to be secreted. The tag they (The Wisconsin Team) designed could not be used as an in fusion tag. The 2 options that were available to us were to either use the Freiburg fusion standard for assembly or use the BBF RFC 28 standard.

We instead added a base at the end of the tag in such a way that when it was fused with the gene of the protein to be secreted, it would be an in-frame after the scar that would result due to standard assembly.

We therefore chose to add a 'C' to the end of the designed export tag to allow it to be fused in frame. This caused an addition of alanine when translated.

We checked the changes in secondary structure that this addition caused by the available online tools like SOPMA and SSpro. No significant changes were predicted.

Source

Oligonucleotides

References

1. https://parts.igem.org/Part:BBa_K130002

2. http://www.jbc.org/content/281/20/13972.long

3. http://2007.igem.org/Freiburg07/report_fusion_parts

4. http://dspace.mit.edu/handle/1721.1/46721

5. http://www.jbc.org/content/282/11/8309.full